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人臍靜脈內(nèi)皮細(xì)胞 HUVEC

人臍靜脈內(nèi)皮細(xì)胞 HUVEC

簡(jiǎn)要描述:青旗(上海)生物技術(shù)發(fā)展有限公司,總部位于上海浦東新區(qū),依托本地高校資源,逐步發(fā)展成為以生物技術(shù)為主的研發(fā)、生產(chǎn)、培訓(xùn)為一體的綜合化產(chǎn)業(yè)平臺(tái),在標(biāo)準(zhǔn)化細(xì)胞庫(kù)建立及細(xì)胞藥物前端模型方面成果顯著。公司生產(chǎn)經(jīng)營(yíng)原代細(xì)胞、細(xì)胞系、ELISA試劑盒、感受態(tài)細(xì)胞和HPLC檢測(cè)等科研產(chǎn)品與服務(wù)。我們秉承對(duì)用戶負(fù)責(zé)的態(tài)度,以對(duì)科研的高度嚴(yán)謹(jǐn),以嚴(yán)格的質(zhì)量控制,為廣大生物醫(yī)學(xué)科研用戶提供更優(yōu)質(zhì)的服務(wù)!

更新時(shí)間:2021-05-26

廠商性質(zhì):生產(chǎn)廠家

瀏覽次數(shù):365

詳情介紹
品牌其他品牌貨號(hào)BFN607200285
規(guī)格T25培養(yǎng)瓶x1 1.5ml凍存管x2供貨周期現(xiàn)貨
主要用途僅供科研應(yīng)用領(lǐng)域醫(yī)療衛(wèi)生,生物產(chǎn)業(yè)

細(xì)胞名稱

人臍靜脈內(nèi)皮細(xì) HUVEC

img1

貨物編碼

BFN607200285

產(chǎn)品規(guī)格

T25培養(yǎng)x1

1.5ml凍存x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37

-198

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研用途                1類

 

培養(yǎng)體系

The base medium for this cell line is F-12K Medium (ATCC 30-2004). To make the complete growth medium, add the following components to 440 mL of the base medium:

 

5 mL of a 10 mg/mL stock heparin solution (prepared from Sigma catalog #H3393) for a final concentration of 0.1 mg/mL heparin in complete growth medium

Dissolve 1 g Heparin in 100 mL basal F-12K and filter to make a 10 mg/mL stock solution

5 mL endothelial cell growth supplement (ECGS; BD Biosciences catalog # 354006)

Rehydrate 1 vial using 5 mL basal F-12K

50 mL fetal bovine serum (FBS; ATCC 30-2020)

培養(yǎng)溫度

37

二氧化碳濃度

5%

簡(jiǎn)介

人臍靜脈內(nèi)皮細(xì)胞HUVEC 源于ATCCCRL-1730).部分文獻(xiàn)稱該細(xì)胞為HUVEC-C。

注釋

Characteristics: Life expectancy of 50 to 60 population doublings (ATCC). 54 PDL (PubMed=28755030).

Characteristics: Contains an integrated copy of HHV-6B (CI-HHV-6B) at the distal end of chromosome 9 (PubMed=28755030).

Doubling time: ~23.5 hours (between passages 18 and 30), ~67 hours (between passages 24 and 27), ~84 hours (between passages 27 and 30), ~100 hours (between passages 32 and 34) (PubMed=28755030).

STR信息

/

參考文獻(xiàn)

PubMed=6333682; DOI=10.1073/pnas.81.20.6413

Hoshi H., McKeehan W.L.

Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture.

Proc. Natl. Acad. Sci. U.S.A. 81:6413-6417(1984)

 

PubMed=28755030; DOI=10.1007/s10616-017-0119-y

Shioda S., Kasai F., Ozawa M., Hirayama N., Satoh M., Kameoka Y., Watanabe K., Shimizu N., Tang H., Mori Y., Kohara A.

The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture.

Cytotechnology 70:141-152(2018)

 

PubMed=29892436; DOI=10.1098/rsos.172472

Shioda S., Kasai F., Watanabe K., Kawakami K., Ohtani A., Iemura M., Ozawa M., Arakawa A., Hirayama N., Kawaguchi E., Tano T., Miyata S., Satoh M., Shimizu N., Kohara A.

Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection.

R. Soc. Open Sci. 5:172472-172472(2018)

青旗(上海)生物技術(shù)發(fā)展有限公司,總部位于上海浦東新區(qū),依托本地高校資源,逐步發(fā)展成為以生物技術(shù)為主的研發(fā)、生產(chǎn)、培訓(xùn)為一體的綜合化產(chǎn)業(yè)平臺(tái),在標(biāo)準(zhǔn)化細(xì)胞庫(kù)建立及細(xì)胞藥物前端模型方面成果顯著。公司生產(chǎn)經(jīng)營(yíng)原代細(xì)胞、細(xì)胞系、ELISA試劑盒、感受態(tài)細(xì)胞和HPLC檢測(cè)等科研產(chǎn)品與服務(wù)。我們秉承對(duì)用戶負(fù)責(zé)的態(tài)度,以對(duì)科研的高度嚴(yán)謹(jǐn),以嚴(yán)格的質(zhì)量控制,為廣大生物醫(yī)學(xué)科研用戶提供更優(yōu)質(zhì)的服務(wù)! 



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