細(xì)胞名稱

人腦髓母細(xì)胞瘤細(xì)胞ONS-76

貨物編碼

BFN60808588

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研      3類

培養(yǎng)體系

DMEM高糖培養(yǎng)基+10%FBS+1%三抗

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人腦髓母細(xì)胞瘤細(xì)胞ONS-76取自2歲女性供體。該細(xì)胞源于JCRB

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Doubling time: 18.6 hours (CelloPub=CLPUB00094; PubMed=2504489; PubMed=2818910); ~24 hours (lot 08192008), ~32 hours (lot 12042014) (JCRB).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突變

Heterozygous for NRAS p.Gln61Arg (c.182A>G) (CCLE; Cosmic-CLP).

TERT c.228C>T (-124C>T); in promoter (PubMed=31068700).

Has no TP53 mutation (CCLE; Cosmic-CLP).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        10,12

D2S1338        17,19

D3S1358        15

D5S818        9,10

D7S820        11,12

D8S1179        10

D13S317        8,13

D16S539        9,10

D18S51        14,21

D19S433        14,15.2

D21S11        29,30.3

FGA        24

Penta D        12,13

Penta E        15,16

TH01        9

TPOX        8

vWA        14,16

參考文獻(xiàn)

PubMed=22951319; DOI=10.1093/jrr/rrs078

Sun L., Moritake T., Zheng Y.W., Suzuki K., Gerelchuluun A., Hong Z., Zenkoh J., Taniguchi H., Tsuboi K.

In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76.

J. Radiat. Res. 54:61-69(2013)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

PubMed=27498314; DOI=10.1016/j.jbiotec.2016.07.028

Ivanov D.P., Coyle B., Walker D.A., Grabowska A.M.

In vitro models of medulloblastoma: Choosing the right tool for the job.

J. Biotechnol. 236:10-25(2016)

PubMed=27812533; DOI=10.1016/j.dib.2016.10.004

Ivanov D.P., Walker D.A., Coyle B., Grabowska A.M.

Data on the number and frequency of scientific literature citations for established medulloblastoma cell lines.

Data Brief 9:696-698(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)